The lyrics describe someone who feels lost, tired, and at their worst until they are comforted and set free by a presence that is referred to as the "one true vine."

Meaning of "One True Vine" by Mavis Staples

The lyrics of "One True Vine" can be interpreted as a metaphor for finding solace, redemption, and salvation in a higher power or a divine force. The imagery of being last in line and feeling dead at first conveys a sense of hopelessness and despair, but the arrival of the one true vine brings renewal and liberation. This presence acts as a source of comfort and trust, guiding the speaker out of darkness and into a place of light and peace. The repeated reference to being set free from a "mighty, mighty fire" can symbolize being rescued from one's own destructive tendencies or inner turmoil. Ultimately, the song expresses a yearning for a deeper connection with this guiding force, hoping to reciprocate the trust and to embody its positive influence.